Related Experiment Video
Updated: Apr 17, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Characterization of bacterial isolates from rubber dump site and their use in biodegradation of isoprene in batch and
Navnita Srivastva1, Awadhesh Kumar Shukla1, Ram Sharan Singh2
1Department of Botany, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
Abstract:
Bacterial isolates from contaminated soil of a waste rubber dumping site were isolated and characterized using biochemical and molecular approaches. Isoprene degradation kinetics in batch mode (isoprene concentration: 100-1000 ppm) revealed the degradation efficiency of isolates as: Pseudomonas sp. (83%)>Alcaligenes sp. (70%)>Klebsiella sp. (68.5%). The most efficient isolate Pseudomonas sp. was finally inoculated in a specifically designed bioreactor system comprising a bioscrubber and a biofilter packed with polyurethane foam connected in series. The bioscrubber and biofilter units when operated in a series showed more than 90% removal efficiency up to the inlet loading rate (IL) of 371.1g/m(3)/h. Maximum elimination capacity (EC) of biofilter was found to be an order of magnitude greater than that for bioscrubber. Oxidative cleavage of the double bond of isoprene has been revealed through IR spectra of the leachate.
More Related Videos
08:21Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
13:38Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture
Published on: May 10, 2013
Related Concept Videos
Production of Antibiotics
Bioplastics
Microbial Bioremediation of Plastics
Microbial Bioremediation of Hydrocarbons
Bioremediation
Batch vs Continuous Culture