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Updated: Apr 17, 2026

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture
Published on: January 19, 2018
Best practices for fluorescence microscopy of the cyanobacterial circadian clock
Susan E Cohen1, Marcella L Erb2, Joe Pogliano2
1Center for Circadian Biology, University of California, San Diego, La Jolla, California, USA; Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
Abstract:
This chapter deals with methods of monitoring the subcellular localization of proteins in single cells in the circadian model system Synechococcus elongatus PCC 7942. While genetic, biochemical, and structural insights into the cyanobacterial circadian oscillator have flourished, difficulties in achieving informative subcellular imaging in cyanobacterial cells have delayed progress of the cell biology aspects of the clock. Here, we describe best practices for using fluorescent protein tags to monitor localization. Specifically, we address how to vet fusion proteins and overcome challenges in microscopic imaging of very small autofluorescent cells.
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