A H+/Ag+ dual-target responsive label-free light-up probe based on a DNA triplex

Area of Science:

• Biochemistry
• Analytical Chemistry
• Molecular Biology

Background:

• Accurate detection of protons (H+) and silver ions (Ag+) is crucial in various scientific fields.
• Existing detection methods may lack selectivity, sensitivity, or require complex procedures.
• Development of simple, cost-effective, and reliable sensors is an ongoing need.

Purpose of the Study:

• To develop a dual-target responsive sensor for label-free fluorescent detection of protons (H+) and silver ions (Ag+).
• To implement an
• OR
• logic gate for simultaneous or independent detection.
• To utilize berberine as a cost-effective and non-toxic fluorescent indicator.

Main Methods:

• Design of a DNA-based sensor capable of forming a triplex structure.
• Partial intercalation of berberine into the DNA triplex upon binding with H+ or Ag+.
• Utilizing the enhanced fluorescence of berberine for signal generation.
• Implementation of an
• OR
• logic gate for signal processing.

Main Results:

• The sensor demonstrated label-free, light-up fluorescent detection of both protons (H+) and silver ions (Ag+).
• The designed silver ion (Ag+) probe exhibited high selectivity and sensitivity.
• The
• OR
• logic gate showed a rapid and reversible response to the presence of H+ and/or Ag+.
• Berberine acted as an effective and non-toxic fluorescent indicator.

Conclusions:

• A novel dual-target responsive sensor based on DNA triplex and berberine was successfully developed.
• The sensor effectively performs label-free, light-up fluorescent detection with an
• OR
• logic gate.
• The system offers a promising platform for practical applications requiring selective and sensitive detection of protons and silver ions.