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Is ursodeoxycholic acid crucial for ischemia/reperfusion-induced ovarian injury in rat ovary?
Ali Akdemir1, Cagdas Sahin, Oytun Erbas
1Department of Obstetrics and Gynecology, Medicine Faculty, Ege University, 35040, Izmir, Turkey.
Purpose:
Ursodeoxycholic acid is frequently used in cholestatic liver diseases. Also, it protects hepatocytes against oxidative stress induced by hydrophobic bile acids. We investigated the anti-oxidative effect of ursodeoxycholic acid on ischemia/reperfusion injury after ovarian de-torsion in rats.
Methods:
We designed five study groups. Group 1 (n = 6): Sham-operated group; group 2 (n = 6): torsion group; group 3 (n = 6): torsion and ursodeoxycholic acid, group 4 (n = 7): torsion/de-torsion group; and group 5 (n = 7): torsion/de-torsion and ursodeoxycholic acid. After that, ovarian samples were obtained and examined histologically and tissue levels of malondialdehyde were measured.
Results:
Follicular degeneration, edema and inflammatory cells were significantly decreased in groups 3 and 5 in comparison with groups 2 and 4. Also, groups 4 and 5 were compared in terms of vascular congestion and hemorrhage and these were found to be significantly decreased in group 5. In addition, levels of malondialdehyde were significantly decreased in groups 3 and 5 in comparison with groups 2 and 4.
Conclusions:
We concluded that ursodeoxycholic acid might be useful to protect the ovary against ischemia and reperfusion injury.
