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Blood and urine 8-iso-PGF2α levels in babies of different gestational ages
Sitao Li1, Hu Hao1, Ping Zhou2
1Department of Neonatology, The Sixth Affiliated Hospital of Sun Yat-Sen University Guangzhou 510655, China.
Insights
This study measured 8-iso-prostaglandin F2α (8-iso-PGF2α) in newborns to assess lipid peroxidation. Pre-term and post-term infants showed higher levels, indicating increased oxidative stress.
Area of Science:
- Neonatal research
- Biomarkers of oxidative stress
- Perinatal medicine
Background:
- Lipid peroxidation is a marker of oxidative stress, potentially impacting neonatal health.
- Understanding oxidative stress levels in newborns is crucial for assessing health and development.
Purpose of the Study:
- To measure 8-iso-prostaglandin F2α (8-iso-PGF2α) levels in umbilical cord blood and urine of infants across a range of gestational ages.
- To determine the relationship between gestational age and lipid peroxidation status in newborns.
- To establish reference ranges for 8-iso-PGF2α in full-term infants.
Main Methods:
- Umbilical cord blood (UCB) and urine samples were collected from infants born between 28 and 43 weeks of gestation.
- Infants were categorized into four groups based on gestational age: 28-32, 33-36, 37-41, and 42-43 weeks.
- 8-iso-PGF2α levels were quantified using enzyme-linked immunosorbent assay (ELISA).
Main Results:
- Full-term infants (37-41 weeks) had UCB and urine 8-iso-PGF2α levels of 130.09 ± 31.73 pg/ml and 27.14 ± 6.73 pg/ml, respectively.
- Pre-term infants (28-36 weeks) exhibited higher UCB levels (188.42-189.37 pg/ml) and slightly elevated urine levels (30.46-32.14 pg/ml).
- Post-term infants (42-43 weeks) showed the highest levels in both UCB (252.01 ± 46.42 pg/ml) and urine (44.00 ± 8.50 pg/ml). A significant correlation was observed between UCB and urine 8-iso-PGF2α levels (r = 0.65, P < 0.01).
Conclusions:
- Reference levels for blood and urine 8-iso-PGF2α were established for normal full-term newborns.
- Urine 8-iso-PGF2α concentrations appear to be a reliable indicator of lipid peroxidation in infants.
- Increased lipid peroxidation was evident in both pre-term and post-term infants, suggesting potential oxidative stress during these periods.
Objective:
We measured cord blood and urine 8-iso-prostaglandin F2α (8-iso-PGF2α) levels in babies of different gestational ages to determine lipid peroxidation status.
Methods:
Babies at gestational ages of 28-43 weeks were divided into group A (28-32 weeks), group B (33-36 weeks), group C (37-41 weeks), and group D (42-43 weeks). 8-iso-PGF2α in umbilical cord blood (UCB) at birth and urine at 6 hours after birth was and tested by ELISA.
Results:
UCB and urine 8-iso-PGF2α levels in group C were 130.09 ± 31.73 pg/ml and 27.14 ± 6.73 pg/ml, respectively. UCB 8-iso-PGF2α levels in group A and B were 188.42 ± 59.34 pg/ml and 189.37 ± 68.46 pg/ml, and urine 8-iso-PGF2α were 32.14 ± 7.32 pg/ml and 30.46 ± 8.83 pg/ml, respectively. Blood and urine 8-iso-PGF2α levels in group D (post-term) were 252.01 ± 46.42 pg/ml and 44.00 ± 8.50 pg/ml. For all babies, UCB and urine iso-PGF2α levels were significantly correlated (r = 0.65, P < 0.01).
Conclusions:
We established blood and urine iso-PGF2α levels in normal full-term babies. Urine 8-iso-PGF2α levels may reflect the extent of lipid peroxidation in babies. In pre-term and post-term babies, there was evidence for increased lipid peroxidation.
