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A system for the application of general anaesthetics and other volatile agents to superfused, isolated tissue
D Girdlestone1, S G Cruickshank, W Winlow
1Department of Physiology, University of Leeds, UK.
Abstract:
1. A delivery system for the application of general anaesthetics or other gaseous and volatile agents to superfused, isolated preparations is described in detail. 2. This system delivers known concentrations of anaesthetic and controls for evaporation and absorption of volatile agents, whilst allowing intracellular electrophysiological recordings to be made from the tissue with minimal disturbance. 3. In particular, this delivery system permits accurate, controlled experiments to be carried out on the neuronal actions of general anaesthetics.