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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Apr 17, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

Quantifying storage material accumulation in tissue sections.

Jonathan D Cooper¹, Helen R E Brooks¹, Hemanth R Nelvagal¹

¹Department of Basic and Clinical Neuroscience, Institute of Psychiatry, Psychology & Neuroscience, King's Health Partners Centre for Neurodegeneration Research, King's College London, London, UK.

Methods in Cell Biology

|February 11, 2015

Summary

This summary is machine-generated.

Quantifying storage burden from lysosomal dysfunction is crucial for disease staging and evaluating therapies. This chapter details methods for preserving tissue integrity while visualizing and quantifying storage material using image analysis.

Keywords:

Assessing therapeutic efficacy Autofluorescence Confocal microscopy Disease progression Immunostaining Lysosomal dysfunction Quantitative analysis Storage material accumulation Thresholding image analysis

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Last Updated: Apr 17, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections

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Published on: May 19, 2022

High-Throughput, Multi-Image Cryohistology of Mineralized Tissues

High-Throughput, Multi-Image Cryohistology of Mineralized Tissues

Published on: September 14, 2016

Area of Science:

Biomedical science
Cell biology
Pathology

Background:

Lysosomal storage diseases (LSDs) involve the accumulation of undegraded material within lysosomes.
Accurate quantification of this storage burden is essential for understanding disease progression and treatment efficacy.
Current biochemical methods may compromise tissue integrity, limiting anatomical context.

Purpose of the Study:

To provide practical methodological recommendations for quantifying lysosomal storage material in tissue sections.
To enable reliable assessment of disease progression and therapeutic responses.
To emphasize the importance of maintaining anatomical integrity during analysis.

Main Methods:

Direct visualization or staining of storage material within tissue sections.
Utilizing image analysis techniques for quantification.
Focusing on methods that preserve the anatomical structure of the tissue sample.

Main Results:

The proposed methods allow for reliable quantification of storage material.
Anatomical integrity of tissue samples is maintained throughout the process.
The approach facilitates both disease staging and therapeutic strategy assessment.

Conclusions:

Effective quantification of lysosomal storage burden is achievable while preserving tissue architecture.
These methods offer a valuable tool for researchers studying lysosomal storage diseases.
The described techniques support the evaluation of therapeutic interventions in LSDs.