Related Experiment Video
Updated: Apr 17, 2026

Induction and Assessment of Exertional Skeletal Muscle Damage in Humans
Published on: December 11, 2016
Acute responses and muscle damage in different high-intensity interval running protocols
T Wiewelhove1, J Fernandez-Fernandez, C Raeder
1Faculty of Sport Science, Ruhr--University, Bochum, Germany - thimo.wiewelhove@ruhr--uni--bochum.de.
High-intensity interval training (HIT) protocols vary in physiological demands and muscle damage. Longer intervals increase cardiovascular response, while sprint protocols cause greater muscle damage and soreness.
Area of Science:
- Exercise Physiology
- Sports Science
- Training Adaptations
Background:
- High-intensity interval training (HIT) is a popular training method.
- Different HIT protocols may elicit distinct physiological responses and muscle damage.
- Optimizing HIT requires understanding the impact of varying interval durations and intensities.
Purpose of the Study:
- To evaluate the acute physiological responses and exercise-induced muscle damage from five distinct HIT protocols.
- To compare the effects of HIT protocols varying in interval duration and intensity, matched to maximal velocity from the 30-15 Intermittent Fitness Test (VIFT).
Main Methods:
- Sixteen well-trained intermittent sport players completed five HIT protocols with varying work/rest ratios and intensities.
- Measurements included blood lactate, pH, creatine kinase (CK), heart rate (HR), session rating of perceived exertion (session-RPE), delayed onset muscle soreness (DOMS), and countermovement jump (CMJ) height.
- Protocols ranged from longer intervals (e.g., 4x4 min at 80% VIFT) to short sprints (e.g., 4x6x5s sprints).
Main Results:
- Longer HIT intervals (P240, P120) elicited higher HR, session-RPE, and lactate, with inverse changes in blood pH.
- Shorter sprint protocols (P5) resulted in the highest lactate concentration and greatest decrease in blood pH.
- 24 hours post-exercise, P5 induced significantly higher CK, DOMS, and reduced CMJ height compared to other protocols.
Conclusions:
- HIT protocols with different interval durations and intensities induce varying physiological and perceptual demands.
- Longer intervals at submaximal intensities enhance acute card circulatory responses.
- Sprint-based HIT protocols lead to the most significant exercise-induced muscle damage and soreness.
More Related Videos
07:40Impact of High-intensity Interval Exercise and Moderate-Intensity Continuous Exercise on the Cardiac Troponin T Level at an Early Stage of Training
Published on: October 10, 2019
08:27A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement
Published on: February 22, 2022
Related Concept Videos
Exercise and Cardiovascular Response
Light to moderate physical activity initiates a series of interconnected responses in the body. The heart rate modestly increases in anticipation of the workout, followed by widespread vasodilation as oxygen consumption by skeletal muscles increases. This results in decreased peripheral resistance, increased capillary blood flow, and accelerated...
Muscle Recovery and Fatigue
Exercise and Muscle Performance
Endurance exercises
Endurance exercises involve running, swimming, or cycling, which require repetitive movements with low force output. When a person engages in endurance exercise, a few noticeable changes occur in their skeletal muscles. For instance, the number of capillaries...
Exercise and Cardiac Output
Sustained exercise increases the muscles' oxygen demand, which can be...
Motor Unit Stimulation
The latent period of contraction marks the onset of excitation-contraction coupling, when the action potential propagates across the sarcolemma, preparing the muscle fibers for contraction. As the fibers enter the contraction phase, the...
Exercise Stress Test
Exercise stress testing, commonly known as a treadmill test, is a noninvasive procedure used to evaluate cardiovascular function and diagnose heart conditions.
Definition
An exercise stress test measures the heart's response to exertion using a treadmill or stationary bicycle. Chest electrodes record the heart's electrical activity through an ECG, and blood pressure is monitored regularly.
Purposes