Collection of peripheral hematopoietic stem/progenitor cells

Area of Science:

• Hematology
• Transplantation Science
• Apheresis Technology

Background:

• Peripheral blood stem cells (PBSCs) are the preferred source for hematopoietic stem cell transplantation (HSCT).
• Optimizing PBSC collection is crucial for successful transplantation outcomes.

Purpose of the Study:

• To evaluate factors influencing peripheral hematopoietic stem/progenitor cell (PBSC) collection efficacy.
• To compare different apheresis machines (Amicus vs. Cobe Spectra), venous access methods (peripheral vein vs. central venous catheter), and apheresis regimens (standard vs. large volume leukapheresis).

Main Methods:

• Retrospective analysis of 169 PBSC collection procedures from healthy donors and patients.
• Procedures were performed between January 2008 and December 2011.
• Data collected on apheresis machine type, venous access, and apheresis regimen.

Main Results:

• Cobe Spectra processed larger blood volumes with higher CD34+ cell collection efficiency and lower RBC contamination compared to Amicus.
• Amicus demonstrated lower platelet (PLT) contamination, minimizing post-procedural PLT count decrease.
• Central venous catheter (CVC) usage allowed higher flow rates and larger blood volumes processed per unit time, with a tendency for lower PLT contamination. Large Volume Leukapheresis (LVL) significantly increased hematopoietic stem cell (HSC) yields, even in poor mobilizers.

Conclusions:

• Individualized management of PBSC collection is essential for optimizing outcomes.
• Institutionally and individually optimized collection strategies can improve transplantation success and reduce financial costs.