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Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity
Published on: April 13, 2021
Saccharothrix tamanrassetensis sp. nov., an actinomycete isolated from Saharan soil
Dalila Boubetra1, Abdelghani Zitouni1, Noureddine Bouras2,1
1Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
Abstract:
Actinomycete strain SA198(T), isolated from a Saharan soil sample of Algeria, was characterized taxonomically by using a polyphasic approach. Chemotaxonomic and morphological characteristics observed suggested that it was a member of the genus Saccharothrix . The 16S rRNA gene sequence analysis confirmed that strain SA198(T) was a member of the genus Saccharothrix and showed a similarity level ranging between 97.5 and 98.9% within species of the genus Saccharothrix , Saccharothrix australiensis being the most closely related. However, DNA-DNA hybridization values between strain SA198(T) and its closest phylogenetic neighbours, the type strains of S. australiensis , Saccharothrix xinjiangensis , Saccharothrix algeriensis and Saccharothrix espanaensis , were clearly below the 70% threshold. Based upon genotypic and phenotypic differences from other members of the genus, a novel species, Saccharothrix tamanrassetensis sp. nov., is proposed, with SA198(T) ( = DSM 45947(T) = CECT 8640(T)) as the type strain.
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