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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Published on: March 19, 2018
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Parabacteroides faecis sp. nov., isolated from human faeces
Mitsuo Sakamoto1, Yoshiki Tanaka2,3, Yoshimi Benno3
1Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
International Journal of Systematic and Evolutionary Microbiology
|February 11, 2015
Summary
A novel bacterial species, Parabacteroides faecis, was identified from human feces using polyphasic taxonomy. This obligate anaerobic, Gram-negative rod represents a new species within the Parabacteroides genus.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- The human gut microbiome harbors diverse bacterial species, many yet to be fully characterized.
- Accurate bacterial classification is crucial for understanding host-microbe interactions and gut health.
Purpose of the Study:
- To taxonomically characterize a novel bacterial strain, designated 157(T), isolated from human feces.
- To determine if strain 157(T) represents a new species within the Parabacteroides genus.
Main Methods:
- Polyphasic taxonomic analysis including physiological, biochemical, and cellular fatty acid profiling.
- Phylogenetic analysis based on 16S rRNA and hsp60 gene sequencing.
- Determination of menaquinone profiles and DNA G+C content.
Main Results:
- Strain 157(T) is an obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative rod.
- 16S rRNA and hsp60 gene sequence analyses indicated strain 157(T) belongs to the genus Parabacteroides, closely related to P. gordonii.
- Distinct cellular fatty acid profiles, menaquinone (MK-9), and DNA G+C content (41.8 mol%) supported its novelty.
Conclusions:
- Strain 157(T) represents a novel species of the genus Parabacteroides.
- The proposed name for this new species is Parabacteroides faecis sp. nov., with strain 157(T) as the type strain.
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