Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MSC-AS1 knockdown inhibits cell growth and temozolomide resistance by regulating miR-373-3p/CPEB4 axis in glioma through PI3K/Akt pathway.

Molecular and cellular biochemistry·2020
Same author

Transcriptome and miRNome Analysis Provide New Insight Into Host Lipid Accumulation, Innate Immunity, and Viral Persistence in Hepatitis C Virus Infection <i>in vitro</i>.

Frontiers in microbiology·2020
Same author

Synergistic pathogenicity in sequential coinfection with fowl adenovirus type 4 and avian orthoreovirus.

Veterinary microbiology·2020
Same author

The Change Trend of Cause of Death in Patients With Stage I Non-Small Cell Lung Cancer After Surgery in US: A Long-Term Follow-Up Study Based on SEER Database.

Cancer control : journal of the Moffitt Cancer Center·2020
Same author

Changes in microstructure and rheological properties of konjac glucomannan/zein blend film-forming solution during drying.

Carbohydrate polymers·2020
Same author

Dux-Mediated Corrections of Aberrant H3K9ac during 2-Cell Genome Activation Optimize Efficiency of Somatic Cell Nuclear Transfer.

Cell stem cell·2020

Related Experiment Video

Updated: Apr 17, 2026

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers
11:42

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers

Published on: June 20, 2019

8.4K

Optical nanoimaging for block copolymer self-assembly.

Jie Yan1, Ling-Xi Zhao, Chong Li

  • 1Wuhan National Laboratory for Optoelectronics and ‡College of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan, Hubei 430074, China.

Journal of the American Chemical Society
|February 11, 2015
PubMed
Summary

This study demonstrates sub-diffraction optical nanoimaging of block copolymer self-assembly using photoswitchable spiropyrans (SPs). These SPs enable high-resolution visualization of microphase structures in polystyrene-block-poly(ethylene oxide) micelles.

More Related Videos

Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives
09:22

Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives

Published on: February 7, 2017

8.4K
Gyroid Nickel Nanostructures from Diblock Copolymer Supramolecules
08:40

Gyroid Nickel Nanostructures from Diblock Copolymer Supramolecules

Published on: April 28, 2014

13.0K

Related Experiment Videos

Last Updated: Apr 17, 2026

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers
11:42

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers

Published on: June 20, 2019

8.4K
Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives
09:22

Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives

Published on: February 7, 2017

8.4K
Gyroid Nickel Nanostructures from Diblock Copolymer Supramolecules
08:40

Gyroid Nickel Nanostructures from Diblock Copolymer Supramolecules

Published on: April 28, 2014

13.0K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Polymer Chemistry

Background:

  • Optical nanoimaging aims to surpass the diffraction limit for higher resolution.
  • Photoswitchable fluorophores offer potential for super-resolution imaging techniques.
  • Block copolymers self-assemble into ordered microstructures, crucial for advanced materials.

Purpose of the Study:

  • To develop a robust optical nanoimaging method for visualizing block copolymer self-assembly.
  • To achieve sub-diffraction resolution imaging of microphase structures in block copolymer micelles.
  • To utilize photoswitchable fluorophores for enhanced contrast and localization.

Main Methods:

  • Assembly of polystyrene-block-poly(ethylene oxide) (PSt-b-PEO) block copolymer micelles.
  • Staining of the hydrophobic polystyrene domains with spiropyrans (SPs).
  • Sequential molecular localization and fluorescence switching of SPs using UV and visible light irradiation.

Main Results:

  • Spiropyrans selectively localized in the hydrophobic polystyrene phase of the micelles.
  • Reversible on-off fluorescence switching of SPs was achieved.
  • Optical nanoimaging of microphase structures with 50-nm resolution was demonstrated.

Conclusions:

  • This method provides a sturdy realization of optical nanoimaging with sub-diffraction resolution for solution self-assembly.
  • Phase-selective distribution of SPs is key to visualizing block copolymer microstructures.
  • The technique offers a powerful tool for studying self-assembled polymer systems.