Related Experiment Video
Updated: Apr 17, 2026
IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
Published on: July 7, 2015
Exploitation and application of a highly sensitive Ru(II) complex-based phosphorescent chemodosimeter for Hg2+ in
Jiaxi Ru1, Xiaoliang Tang, Zhenghua Ju
1Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering and ‡School of Life Sciences, Lanzhou University , Lanzhou, 730000, China.
Abstract:
A novel Ru(II) complex-based phosphorescent probe Rubpy-1 was designed and synthesized conveniently by incorporating of chemodosimeter into the luminophor, which exhibits good water solubility, longer excitation wavelength, and rapid turn-on phosphorescent response only toward Hg(2+) in aqueous system under physiological pH. The spectral response mechanism and Hg(2+)-promoted structure change of the chemodosimeter were analyzed in detail by theoretical calculations and electrospray ionization mass spectrometry. When time-resolved photoluminescence techniques were used, the Rubpy-1 could eliminate effectively the signal interference from the short-lived background fluorescence in complicated media, accompanied by the significant improvement of the signal-to-noise ratio and the accuracy of the detection. Furthermore, Rubpy-1 showed low cytotoxicity and excellent membrane permeability toward living cells, which was successfully applied to monitor intracellular Hg(2+) effectively by confocal luminescence imaging.
