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Related Experiment Video

Updated: Apr 17, 2026

Author Spotlight: Insights into the Effect of Ischemia Reperfusion on Lung Transplantation
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Multipotent adult progenitor cells decrease cold ischemic injury in ex vivo perfused human lungs: an initial pilot

Saverio La Francesca1, Anthony E Ting2, Jason Sakamoto3

  • 1Cardiac Surgery and Cardiopulmonary Transplantation, DeBakey Heart and Vascular Center, The Houston Methodist, Houston, TX USA ; Harvard Apparatus Regenerative Technology, Inc, Holliston, MA USA.

Transplantation Research
|February 12, 2015
PubMed
Summary
This summary is machine-generated.

Intratracheal administration of multipotent adult progenitor cells (MAPCs) during donor lung processing reduced inflammation and injury. This novel approach shows promise for improving lung transplant outcomes by mitigating ischemia-reperfusion injury.

Keywords:
Cell therapyIschemia-reperfusion injuryLung transplantationMesenchymal stromal cell

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Area of Science:

  • Regenerative Medicine
  • Transplantation Immunology
  • Cell Therapy

Background:

  • Primary graft dysfunction (PGD) is a major complication after lung transplantation, leading to significant morbidity and mortality.
  • Ischemia-reperfusion injury (IRI) during organ preservation is a key contributor to PGD.
  • Multipotent adult progenitor cells (MAPCs) possess anti-inflammatory properties that may mitigate IRI.

Purpose of the Study:

  • To investigate the efficacy of intratracheal MAPC instillation in reducing lung injury and inflammation.
  • To evaluate MAPC treatment in an ex vivo human lung explant model simulating cold storage and reperfusion.

Main Methods:

  • Donor lungs underwent 8 hours of cold storage.
  • Non-HLA-matched allogeneic MAPCs were instilled into one lung lobe, with vehicle control in the contralateral lobe.
  • Lungs were reperfused and ventilated for 4 hours, followed by assessment of histologic injury and inflammatory markers.

Main Results:

  • MAPC-treated lung lobes showed significantly reduced histologic injury compared to vehicle-treated lobes.
  • Bronchoalveolar lavage fluid (BALF) analysis revealed decreased inflammation in MAPC-treated lobes.
  • Consistent reduction in inflammatory markers was observed across all MAPC-treated lung segments.

Conclusions:

  • Intratracheal administration of MAPCs during donor lung processing effectively decreased markers of cold ischemia-induced lung injury.
  • These findings suggest a potential therapeutic strategy using MAPCs to improve lung preservation and reduce PGD.
  • Further studies are warranted to validate these promising results in clinical settings.