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Updated: Apr 17, 2026

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

Local pH tracking in living cells.

Chieh-Jui Tsou¹, Chih-Hao Hsia, Jia-Yin Chu

¹Department of Chemistry, National Taiwan University, Taipei, Taiwan 106. cymou@ntu.edu.tw.

|February 13, 2015

Summary

This summary is machine-generated.

Researchers developed fluorescent mesoporous silica nanoparticles (FMSNs) for simultaneous 3D tracking and pH sensing in cells. This breakthrough enables real-time monitoring of nanoparticle environments during cellular uptake, aiding drug delivery system design.

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Area of Science:

Nanotechnology
Cell Biology
Biomedical Engineering

Background:

Accurate monitoring of nanoparticle behavior within cells is crucial for developing effective drug delivery systems.
Understanding the local pH changes during cellular uptake is essential for optimizing nanoparticle design and function.

Purpose of the Study:

To demonstrate continuous and simultaneous 3D single-particle movement and local pH detection in HeLa cells.
To develop and validate fluorescent mesoporous silica nanoparticles (FMSNs) for precise intracellular measurements.

Main Methods:

Synthesized FMSNs by co-condensing pH-sensitive and reference dyes with silica/surfactant.
Combined FMSNs with single-particle tracking (SPT) for 3D movement analysis.
Utilized ratiometric measurements for long-term, stable pH detection with high precision (<0.3 pH unit).

Main Results:

Achieved 3D single-particle tracking with ~10 nm precision.
Monitored real-time pH variations around FMSNs during endocytosis.
Observed early-stage endocytosis with acidification and low FMSN mobility.
Observed late-stage endocytosis with basification and high FMSN mobility.

Conclusions:

Successfully monitored local pH changes surrounding nanoparticles during cellular uptake.
Provided critical insights into nanoparticle-cell interactions and intracellular environments.
Established a novel method for designing efficient drug delivery nanosystems based on real-time cellular monitoring.