Rapid determination of catecholamines in urine samples by nonaqueous microchip electrophoresis with LIF detection
Hongmei Hu1, Yuanming Guo, Tiejun Li
1Key Lab of Mariculture and Enhancement of Zhejiang Province, Marine Fishery Institute of Zhejiang Province, Zhoushan, 316021, China.
Abstract:
Nonaqueous microchip electrophoresis (NAMCE), which makes use of an organic medium instead of a conventional aqueous buffer solution, is a promising separation method for analytical chemistry due to the enhanced solubility of hydrophobic analytes and tailored selectivity of separation. Here, we describe an NAMCE with LIF detection combined with a pump-free negative pressure sampling device for rapid determination of catecholamines (CAs) in urine samples, and the whole analysis time (including sampling time and separation time) was less than 1 min.
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