Related Experiment Videos
Enhanced expression of long noncoding RNA CARLo-5 is associated with the development of gastric cancer
Yan Zhang1, Mingzhe Ma2, Weiyong Liu3
1Department of Gastroenterology, Renmin Hospital of Wuhan University Wuhan, Hubei, China ; Department of Gastroenterology, Yijishan Hospital Affiliated to Wannan Medical College Wuhu, Anhui, China.
Abstract:
The identification of cancer-associated long non-coding RNAs and the investigation of their molecular and biological functions are vital for understanding the molecular biology and progression of cancer. The CARLo-5, a newly identified long non-coding RNA, was found to be upregulated in colon cancer. However, little is known about its role in gastric cancer. In the present study, a great upregulation of CARLo-5 was observed in gastric cancer compared to paired adjacent normal tissues. Knockdown of CARLo-5 in gastric cancer cell lines significantly inhibited the cell proliferation via inducing G0/G1 cell-cycle arrest and apoptosis. Furthermore, ERK/MAPK pathway was found to be inactivated in the gastric cells after CARLo-5 knockdown. These results indicated that CARLo-5 might serve as a pro-oncogenic lncRNA that promotes proliferation of gastric cancer and activates the ERK/MAPK pathway.
Related Concept Videos
lncRNA - Long Non-coding RNAs
lncRNA - Long Non-coding RNAs
Non-LTR Retrotransposons
MicroRNAs
MicroRNAs