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A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

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The correlation between hepatitis C core antigen and hepatitis C virus RNA levels with respect to human

Vo Duy Thong1, Srunthron Akkarathamrongsin, Anchalee Avihingsanon

  • 1Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Intervirology
|February 14, 2015
PubMed

Insights

Serum hepatitis C virus core antigen (HCVcAg) levels strongly correlate with HCV RNA, especially in coinfected patients. This HCV marker may be useful in resource-limited settings.

Area of Science:

  • Hepatology
  • Virology
  • Infectious Diseases

Background:

  • Serum hepatitis C virus core antigen (HCVcAg) correlates with HCV RNA in monoinfected individuals.
  • Limited data exist on HCVcAg in HCV/HIV coinfected patients.

Purpose of the Study:

  • To compare HCVcAg measurement against HIV status, HCV genotypes, IFNL4 polymorphism, and clinical parameters.
  • To evaluate HCVcAg as a potential alternative to HCV RNA assays.

Main Methods:

  • Analysis of an untreated cohort: 104 HCV monoinfected and 85 HCV/HIV coinfected patients.
  • Serum HCVcAg measured via chemiluminescent microparticle immunoassay.
  • IFNL4 polymorphism ss469415590 identified by real-time PCR.

Main Results:

  • HCVcAg strongly correlated with HCV RNA (r = 0.889, p < 0.001), particularly in coinfected patients.
  • Mean HCVcAg was higher in coinfected vs. monoinfected patients.
  • HCVcAg levels associated with IFNL4 TT/TT genotype but not ALT or liver stiffness.

Conclusions:

  • HCVcAg shows excellent correlation with HCV RNA, especially in HCV/HIV coinfection.
  • HCVcAg may be influenced by IFNL4 polymorphism.
  • HCVcAg testing offers a potential alternative to HCV RNA assays in resource-limited settings.
Abstract

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