Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TyG Index and Frailty as Composite Biomarkers of Cardiometabolic Risk and Mortality Across CKM Stages 0-3.

Metabolites·2026
Same author

Buried Interface-Driven Synergistic Homogenization of Self-Assembled Monolayer and Wide-Bandgap Perovskite for Efficient and Stable Solar Cells.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

From click to green choice: the influence of social media content type on green consumption intention.

Frontiers in psychology·2026
Same author

Australian Climate Leadership in 2026: COP-Out or Step-Up for Health?

The Medical journal of Australia·2026
Same author

Sustained modulation of microbial communities by high-performance DCOIT microcapsules for enhanced antifouling and anticorrosion performance of submerged marine infrastructure.

Colloids and surfaces. B, Biointerfaces·2026
Same author

A Biomimetic Micro-Nano System Maximizing 'Active Transport and Retention' Effect in Solid Tumors.

Small (Weinheim an der Bergstrasse, Germany)·2026

Related Experiment Video

Updated: Apr 17, 2026

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
08:16

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

Published on: August 13, 2021

3.3K

Development of the zebrafish mesonephros.

Cuong Q Diep1, Zhenzhen Peng, Tobechukwu K Ukah

  • 1Department of Medicine, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts; Department of Medicine, Harvard Medical School, Boston, Massachusetts; Kidney Program, Harvard Stem Cell Institute, Cambridge, Massachusetts; Department of Biology, Indiana University of Pennsylvania, Indiana, Pennsylvania.

Genesis (New York, N.Y. : 2000)
|February 14, 2015
PubMed
Summary

Zebrafish mesonephros development involves new nephrons forming on pronephric tubules. The pronephros acts as a scaffold, guiding mesonephros fusion to create the adult kidney structure.

Keywords:
kidneynephronpronephros

More Related Videos

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning
10:05

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Published on: April 7, 2016

11.3K
Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney
08:53

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

Published on: August 9, 2014

45.8K

Related Experiment Videos

Last Updated: Apr 17, 2026

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
08:16

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

Published on: August 13, 2021

3.3K
Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning
10:05

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Published on: April 7, 2016

11.3K
Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney
08:53

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

Published on: August 9, 2014

45.8K

Area of Science:

  • Developmental Biology
  • Comparative Physiology
  • Renal Biology

Background:

  • The vertebrate kidney, composed of nephrons, is crucial for waste excretion and osmoregulation.
  • Zebrafish develop an embryonic kidney (pronephros) and an adult kidney (mesonephros).
  • Mesonephros development remains less understood compared to pronephros development.

Purpose of the Study:

  • To characterize mesonephros development in zebrafish during larval metamorphosis.
  • To investigate the cellular origins and patterning of mesonephric nephrons.
  • To elucidate the relationship between pronephros and mesonephros formation.

Main Methods:

  • Zebrafish model organism.
  • Analysis of renal transcription factor gene expression (wt1b, pax2a, lhx1a).
  • Histological and imaging techniques to observe nephron development and fusion.

Main Results:

  • Mesonephric nephrons arise from progenitor cells expressing key renal transcription factors.
  • These new nephrons develop atop the pronephric tubules, exhibiting similar segmentation.
  • The pronephros serves as a structural scaffold for the developing mesonephros.

Conclusions:

  • The pronephros guides the formation and integration of the mesonephros.
  • Nephron fusion with pronephric tubules is essential for the adult zebrafish kidney architecture.
  • This study provides insights into vertebrate kidney development and regeneration.