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Updated: Apr 17, 2026
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Rapid detection of ochratoxin A on membrane by dot immunogold filtration assay
Weifeng Chen1, Yucui Jin1, Aiping Liu2
1College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Background:
Ochratoxin A (OTA), a widely distributed mycotoxin produced by certain species of Aspergillus and Penicillium, has been identified as a carcinogenic, hepatotoxic, teratogenic, nephrotoxic and immunotoxic toxin. To reduce the risk of OTA contamination, a rapid, inexpensive, suitable and on-site assay for its detection is required.
Results:
In this study a dot immunogold filtration assay (DIGFA) of OTA on high-flow nitrocellulose membrane was developed. Firstly colloidal gold was synthesized and colloidal gold-polyclonal antibody (PcAb) conjugates against OTA were prepared at the optimal colloidal gold-labeled pH value and package amount. Then the colloidal gold-PcAb conjugates were used to develop the OTA DIGFA. The results demonstrated a visual detection limit of approximately 10 ng mL(-1) OTA. In addition, this method had no cross-reaction with zearalenone, aflatoxin B1 or citrinin.
Conclusion:
These results indicated that the developed DIGFA could be applied for the actual detection of samples without complicated steps.
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01:20Immunogold Electron Microscopy
01:15Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.