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Related Experiment Video

Updated: Apr 17, 2026

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

Published on: December 22, 2014

TRPM3 expression in mouse retina.

R Lane Brown¹, Wei-Hong Xiong², James H Peters³

¹Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, Washington, United States of America; WWAMI Medical Education Program, Washington State University, Pullman, Washington, United States of America.

|February 14, 2015

Summary

This summary is machine-generated.

Transient receptor potential melastatin 3 (TRPM3) channels are present in retinal ganglion cells and mediate responses to pregnenolone sulfate. TRPM3 is not essential for basic visual processing in mice.

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Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

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Published on: February 13, 2021

Area of Science:

Neuroscience
Molecular Biology
Vision Science

Background:

Transient receptor potential (TRP) channels are vital for sensory transduction.
TRPM1 is crucial for ON-bipolar cell light responses in the retina.
The function of TRPM3 in the retina remains largely unknown.

Purpose of the Study:

To investigate the role of TRPM3 in the mouse retina.
To determine the cellular localization and function of TRPM3 in retinal ganglion cells.

Main Methods:

Immunohistochemistry to localize TRPM3 in retinal layers.
Electroretinography (ERG) to assess visual function in TRPM3 knockout mice.
Calcium imaging and patch-clamp electrophysiology on isolated retinal ganglion cells.

Main Results:

TRPM3 is localized to the inner plexiform layer and retinal ganglion cells, with higher expression in the OFF sublamina.
ERG recordings from TRPM3 knockout mice showed normal visual responses.
TRPM3 agonists stimulated calcium influx and currents in wild-type retinal ganglion cells, but not in TRPM3 knockout cells.
These agonist-induced responses were blocked by a TRPM3 antagonist.

Conclusions:

TRPM3 is expressed in retinal ganglion cells and contributes to their response to specific agonists like pregnenolone sulfate.
TRPM3 does not appear to play a significant role in the primary visual processing pathways detected by ERG.
TRPM3 represents a potential target for modulating retinal ganglion cell activity.