Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.8K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.8K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

13.9K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
13.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrophilic interaction chromatography and tandem mass spectrometry for the characterization of methylated oligoribonucleotides.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Surface activation and functionalization with aryl-diazonium chemistry enable biomolecules grafting on 3D printed carbon-based electrodes.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same author

Solution-based studies on the contact between the complement receptor 3 ligand-binding domain and simvastatin.

Biochimica et biophysica acta. Proteins and proteomics·2025
Same author

Loop-mediated isothermal amplification (LAMP) assay coupled with gold nanoparticles for colorimetric detection of Trichoderma spp. in Agaricus bisporus cultivation substrates.

Scientific reports·2024
Same author

Investigation of the Affinity of Aptamers for Bacteria by Surface Plasmon Resonance Imaging Using Nanosomes.

ACS applied materials & interfaces·2024
Same author

Warburg-associated acidification represses lactic fermentation independently of lactate, contribution from real-time NMR on cell-free systems.

Scientific reports·2023

Related Experiment Video

Updated: Apr 17, 2026

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
12:07

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

14.5K

Methylene blue phosphoramidite for DNA labelling.

Gabriel De Crozals1, Carole Farre, Monique Sigaud

  • 1Institut des sciences analytiques, CNRS/Université de Lyon. 5, rue de la Doua, 69100 Villeurbanne, France. carole.chaix@isa-lyon.fr.

Chemical Communications (Cambridge, England)
|February 14, 2015
PubMed
Summary

Researchers synthesized a novel methylene blue (MB) phosphoramidite for DNA synthesis. This breakthrough enables the creation of new MB-labeled DNA probes with confirmed electrochemical and optical properties.

More Related Videos

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.7K
Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
11:31

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

10.6K

Related Experiment Videos

Last Updated: Apr 17, 2026

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
12:07

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

14.5K
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.7K
Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
11:31

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

10.6K

Area of Science:

  • Organic Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Methylene blue (MB) is a versatile redox indicator and photosensitizer.
  • Oligonucleotide synthesis is crucial for molecular biology and diagnostics.
  • Developing efficient labeling methods for oligonucleotides is an ongoing challenge.

Purpose of the Study:

  • To synthesize a novel methylene blue (MB) phosphoramidite derivative.
  • To enable the incorporation of MB into DNA sequences via solid-phase synthesis.
  • To characterize the properties of MB-modified oligonucleotides.

Main Methods:

  • Chemical synthesis of a phosphoramidite derivative of methylene blue.
  • Solid-phase DNA synthesis incorporating the MB phosphoramidite.
  • Electrochemical and optical characterization of the modified oligonucleotides.

Main Results:

  • Successful synthesis of the MB phosphoramidite derivative.
  • Demonstration of its suitability for DNA solid-phase synthesis.
  • Confirmation of the electrochemical and optical properties of MB-modified DNA.

Conclusions:

  • The developed MB phosphoramidite is a key advancement for creating MB-labeled DNA probes.
  • This facilitates the design and application of novel oligonucleotide-based sensors and tools.
  • It opens new avenues for MB applications in molecular biology and diagnostics.