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MicroRNA-572 improves early post-operative cognitive dysfunction by down-regulating neural cell adhesion molecule 1
Xiya Yu1, Shupeng Liu2, Jinbao Li1
1Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
Abstract:
Post-operative cognitive dysfunction (POCD) is a commonly-seen postoperative complication in elderly patients. However, the underlying mechanisms of POCD remain unclear. miRNAs, which are reported to be involved in the pathogenesis of the nervous system diseases, may also affect POCD. In this study, miRNA microarray technology was used to analyze the circulating miRNA expression profile of POCD patients. Among the altered miRNAs, miR-572 had the greatest decrease, which was also verified in vivo in rat POCD model. Further analysis found that miR-572 could regulate the expression of NCAM1 in the hippocampal neurons and interfering miR-572 expression could facilitate the restoration of cognitive function in vivo. Moreover, clinical correlation analysis found that the miR-572 expression was associated with the incidence of POCD. Collectively, miR-572 is involved in the development and restoration of POCD and it may serve as a biological marker for early diagnosis of POCD.
Insights
Post-operative cognitive dysfunction (POCD) is a common complication in older adults. This study identifies miR-572 as a key factor in POCD development and recovery, suggesting its potential as an early diagnostic biomarker.
Area of Science:
- Neuroscience
- Molecular Biology
- Geriatrics
Background:
- Post-operative cognitive dysfunction (POCD) is a frequent complication in elderly patients.
- The precise mechanisms underlying POCD remain largely unknown.
- MicroRNAs (miRNAs) are implicated in nervous system diseases and may play a role in POCD.
Purpose of the Study:
- To investigate the role of circulating miRNAs in the pathogenesis of POCD.
- To identify potential biomarkers for early POCD diagnosis.
- To explore the therapeutic potential of targeting specific miRNAs in POCD.
Main Methods:
- miRNA microarray technology was employed to analyze circulating miRNA expression profiles in POCD patients.
- In vivo rat models of POCD were established to validate findings.
- Expression levels of miR-572 and its target gene NCAM1 were assessed in hippocampal neurons.
- Intervention studies were performed to evaluate the effect of modulating miR-572 on cognitive function.
Main Results:
- A distinct circulating miRNA expression profile was identified in POCD patients.
- miR-572 showed the most significant decrease among altered miRNAs and was validated in a rat POCD model.
- miR-572 was found to regulate NCAM1 expression in hippocampal neurons.
- Interfering with miR-572 expression promoted the restoration of cognitive function in vivo.
- Clinical correlation analysis revealed an association between miR-572 expression levels and POCD incidence.
Conclusions:
- miR-572 is critically involved in both the development and recovery phases of POCD.
- Modulating miR-572 offers a potential therapeutic strategy for POCD.
- miR-572 may serve as a valuable biomarker for the early diagnosis of POCD.
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