Hyperspectral time-resolved wide-field fluorescence molecular tomography based on structured light and single-pixel...

Area of Science:

• Biomedical Optics
• Optical Imaging
• Biophotonics

Background:

• Diffuse optical tomography (DOT) is a powerful non-invasive imaging technique.
• Accurate quantification of fluorescent probes in vivo remains challenging.
• Existing DOT systems often lack spectral and temporal resolution for multiplexed imaging.

Purpose of the Study:

• To introduce a new time-resolved fluorescence diffuse optical tomography (TR-FDOT) platform.
• To demonstrate the system's capability for 4D (space, time, spectra) data acquisition.
• To enable quantitative mapping of multiple fluorophores in scattering media.

Main Methods:

• Utilized wide-field structured illumination and single-pixel detection.
• Employed two digital micro-mirror devices for independent pattern generation.
• Integrated a 16-channel spectrophotometer for hyperspectral time-resolved data capture.

Main Results:

• Successfully acquired dense 4D tomographic datasets.
• Demonstrated feasibility of 3D quantitative multiplexed fluorophore concentration mapping.
• Characterized the main system performance parameters.

Conclusions:

• The developed TR-FDOT platform offers advanced capabilities for biomedical research.
• This system facilitates precise quantification of multiple fluorescent agents in turbid media.
• Opens new avenues for in vivo molecular imaging and diagnostics.