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Somatostatin increases intracellular Ca2+ concentration in cultured rat hippocampal neurons
R Miyoshi1, S Kito, S Katayama
1Third Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Brain Research
|June 12, 1989
Abstract:
Changes of intracellular Ca2+ concentration [( Ca2+]i) in response to somatostatin were measured in cultured rat hippocampal neurons on a single cell basis by fura-2 fluorometry. Somatostatin increased [Ca2+]i dose-dependently and this effect was completely blocked in either Ca2+-depleted medium or LaCl3-containing medium. In addition, omega-conotoxin GVIA completely inhibited the effect of somatostatin. Our results indicate that somatostatin receptors couple with N-type voltage-sensitive Ca2+ channels in cultured rat hippocampal neurons.