Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same author

Defect-enhanced photothermal catalysis over ZnO for efficient depolymerization of waste polyester into high-purity BHET.

Chemical communications (Cambridge, England)·2026
Same author

Xueshuantong injection alleviates ischemic heart failure via targeting PFKP-mediated endothelial-to-mesenchymal transition.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Structural conservation and ion selectivity adaptation of the mechanically activated PIEZO channel.

Neuron·2026
Same author

IgG Propels Atherosclerosis by Noncanonically Activating Macrophages.

bioRxiv : the preprint server for biology·2026
Same author

Uncoupling of apnea-hypopnea index and oxygen desaturation in als: characterization and diagnostic implications of an "AHI-SpO₂ dissociation" phenotype.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2026

Related Experiment Video

Updated: Apr 17, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.8K

Alignment of direct detection device micrographs using a robust Optical Flow approach.

Vahid Abrishami1, Javier Vargas1, Xueming Li2

  • 1Biocomputing Unit, Centro Nacional de Biotecnología-CSIC, C/ Darwin 3, 28049 Madrid, Spain.

Journal of Structural Biology
|February 15, 2015
PubMed
Summary
This summary is machine-generated.

Beam-induced motion (BIM) in cryo-EM is corrected using a novel image-level method. This approach compensates for blurring before particle picking, improving image quality for structural analysis.

Keywords:
Beam induced motionDirect detection devicesElectron microscopySingle particle analysis

More Related Videos

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

10.4K
Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques
10:53

Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques

Published on: March 12, 2019

7.7K

Related Experiment Videos

Last Updated: Apr 17, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.8K
Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

10.4K
Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques
10:53

Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques

Published on: March 12, 2019

7.7K

Area of Science:

  • Structural Biology
  • Biophysics
  • Microscopy

Background:

  • Direct detection devices in cryo-electron microscopy (cryo-EM) reveal significant beam-induced motion (BIM) in specimens.
  • This motion introduces blurring, compromising the resolution and quality of structural data.

Purpose of the Study:

  • To develop and implement an image-level method for correcting beam-induced motion (BIM) in cryo-EM data.
  • To improve image quality by compensating for in-plane BIM blurring before particle picking.

Main Methods:

  • A robust Optical Flow (OF) approach was employed for efficient, local motion correction at the image level.
  • The method was adapted to handle both local BIM patterns (direct OF application) and global movements (preceded by global motion correction).
  • Spatial analysis of Optical Flow was utilized for quantitative assessment of BIM patterns.

Main Results:

  • The developed method effectively corrects in-plane BIM blurring, producing integrated images with improved clarity.
  • Optical Flow demonstrated high efficiency in correcting local movements, particularly beneficial for datasets like Falcon II.
  • A combined approach of global motion correction followed by OF proved advantageous for global BIM patterns.

Conclusions:

  • The new image-level BIM correction method enhances cryo-EM data quality by mitigating motion-induced blurring.
  • The software implementing this approach is available in XMIPP, facilitating its adoption in structural biology research.
  • This technique offers a valuable tool for quantitative analysis of beam-induced motion in cryo-EM imaging.