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Calmodulin-stimulated particulate guanylate cyclase in crayfish hepatopancreas
W Riediger1, H G Hergenhahn, D Sedlmeier
1Institut für Zoophysiologie, Universität Bonn, B.R.D.
The International Journal of Biochemistry
|January 1, 1989
Abstract:
1. A particulate guanylate cyclase from crayfish hepatopancreas membranes was investigated with respect to its dependence on Ca2+ and calmodulin. Addition of Ca2+ to EGTA-treated membranes increased cyclase activity by 100%. 2. Calmodulin stimulated the activity about 5-fold. 3. This effect could be abolished by the calmodulin antagonist compound 48/80. 4. These results present evidence that the particulate guanylate cyclase of crayfish hepatopancreas is a Ca2+/calmodulin-dependent enzyme. 5. The implications of this observation upon glycogen metabolism of crustaceans are discussed.