Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Metabolism01:19

Regulation of Metabolism

12.6K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
12.6K
Methods of Medium Optimization01:28

Methods of Medium Optimization

63
Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...
63
Bioreactor Controls-III01:22

Bioreactor Controls-III

57
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
57
Upstream Processing01:27

Upstream Processing

89
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
89
Operon Model01:23

Operon Model

2.3K
The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
2.3K
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

1.3K
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Glycine Betaine on Chilling Injury in Eggplant Peel Revealed by FTIR Spectroscopy.

Foods (Basel, Switzerland)·2026
Same author

Spatial Patterns and Diversity of the Genus <i>Agave</i> in the Southern Iberian Peninsula: The Role of Anthropogenic Drivers in the Expansion of <i>Agave americana</i>.

Plants (Basel, Switzerland)·2026
Same author

Awareness and risk perception of bovine brucellosis among subsistence and medium-large scale farmers, and veterinarians: Insights from focus groups in Ecuador.

Preventive veterinary medicine·2025
Same author

Discovery of a Potent, Selective, and Brain-Penetrant Checkpoint Kinase 1 Inhibitor, BEN-28010, for the Treatment of Glioblastoma.

Journal of medicinal chemistry·2025
Same author

A Multiple-Model-Informed Drug-Development Approach for Optimal Regimen Selection of an Oncolytic Virus in Combination With Pembrolizumab.

CPT: pharmacometrics & systems pharmacology·2025
Same author

Multi-sensorial perceptions of risk: the aesthetics behind (muco)cutaneous leishmaniasis-related stigma in Ecuador.

Anthropology & medicine·2023
Same journal

Advanced glucose control strategies leveraging Raman spectroscopy for optimized mammalian cell culture manufacturing.

Biotechnology progress·2026
Same journal

Mechanistic deconvolution of BSA size variants by constrained Raman pseudo-Voigt hard modeling during anion-exchange chromatography.

Biotechnology progress·2026
Same journal

Status and future of recombinant adeno-associated virus vector manufacturing.

Biotechnology progress·2026
Same journal

Multifaceted algae as an ingredient in alternative meat formulations.

Biotechnology progress·2026
Same journal

In-line Raman spectroscopy real-time glucose prediction method for commercial pneumococcal vaccine drug substance fermentation manufacturing process control.

Biotechnology progress·2026
Same journal

Prolonged autophagy induction correlates with host cell protein reduction in CHO cell culture.

Biotechnology progress·2026
See all related articles

Related Experiment Video

Updated: Apr 17, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.9K

A modular modulation method for achieving increases in metabolite production.

Luis Acerenza1, Pablo Monzon2, Fernando Ortega3

  • 1Systems Biology Laboratory, Faculty of Sciences, Universidad de la República, Iguá 4225, Montevideo, 11400, Uruguay.

Biotechnology Progress
|February 17, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces a modular modulation method to identify key genetic targets for enhancing metabolite production in overproducing strains. This approach aids in developing more systematic metabolic engineering strategies.

Keywords:
metabolic engineeringmetabolite productionmodularizationsynthetic biologysystems biology

More Related Videos

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.5K
Light-Controlled Fermentations for Microbial Chemical and Protein Production
08:37

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

4.9K

Related Experiment Videos

Last Updated: Apr 17, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.9K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.5K
Light-Controlled Fermentations for Microbial Chemical and Protein Production
08:37

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

4.9K

Area of Science:

  • Metabolic Engineering
  • Synthetic Biology
  • Biotechnology

Background:

  • Optimizing microbial strains for high-yield metabolite production is crucial but challenging.
  • Current genetic manipulation strategies often lack systematic approaches for identifying key targets.

Purpose of the Study:

  • To develop a modular modulation method for identifying critical genetic manipulation steps to increase metabolite production.
  • To provide a systematic framework for metabolic engineering of overproducing strains.

Main Methods:

  • Metabolic network modularization into two interconnected modules.
  • Modulation of module activity via auxiliary rates affecting linking metabolites.
  • Mathematical formulation using matrix representation for broad applicability.

Main Results:

  • The method is applicable to metabolic networks of any size and complexity, regardless of reaction kinetics.
  • It accommodates various conservation relationships and inter-module interactions.
  • The approach can be integrated with other methods for refined optimization.

Conclusions:

  • The modular modulation method offers a systematic approach to metabolic engineering.
  • It facilitates the identification of key genetic targets for enhanced metabolite production.
  • This procedure can advance metabolic engineering towards a more predictable and efficient practice.