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A mouse chromosome 17 gene encodes a testes-specific transcript with unusual properties
N Sarvetnick1, J Y Tsai, H Fox
1Cold Spring Harbor Laboratory, NY 11724.
Immunogenetics
|January 1, 1989
Summary
A novel mouse gene, D17Si11, found on chromosome 17, is uniquely expressed in testes. Its transcript contains unusual DNA structures and a large complementary open reading frame, suggesting a potential regulatory role.
Area of Science:
- Genetics
- Molecular Biology
- Genomics
Background:
- A novel mouse gene, D17Si11, has been identified on chromosome 17.
- This gene expresses a major transcript predominantly found in testicular tissue.
Purpose of the Study:
- To characterize the novel mouse gene D17Si11 and its transcript.
- To investigate the unique sequence features and potential functions of D17Si11.
Main Methods:
- Gene mapping to locate D17Si11 on chromosome 17.
- Sequence analysis of the D17Si11 locus and its transcript.
- Bioinformatic analysis to identify unusual sequence features and open reading frames.
Main Results:
- D17Si11 was mapped to the central region of chromosome 17.
- The transcript exhibits unusual features, including alternating purine-pyrimidine sequences capable of forming Z-DNA-like structures.
- A long open reading frame was identified in the complementary strand, significantly larger than in the transcript itself.
Conclusions:
- D17Si11 possesses unique structural characteristics within its transcript.
- The presence of a large open reading frame on the complementary strand suggests potential regulatory functions.
- Further research is needed to determine the specific function of D17Si11 in mammalian testes.