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CD44 contributes to T cell activation.

S Huet1, H Groux, B Caillou

  • 1Laboratoire d'Immunologie des Tumeurs de l'Enfant, Institut Gustave-Roussy, Villejuif, France.

Journal of Immunology (Baltimore, Md. : 1950)
|August 1, 1989
PubMed
Summary
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The CD44 molecule, involved in lymphocyte adhesion, also delivers T cell activation signals. Blocking CD44 enhances T cell proliferation, suggesting a distinct signaling pathway from CD28.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The CD44 molecule mediates lymphocyte adhesion to high endothelial venules (HEV).
  • The role of CD44 in T cell activation signaling is not fully understood.

Purpose of the Study:

  • To investigate the involvement of CD44 in T cell activation signaling.
  • To characterize the signaling pathway mediated by CD44.

Main Methods:

  • Production of a CD44 monoclonal antibody (mAb), H90, that blocks lymphocyte binding to HEV.
  • Assessing T cell proliferation ([3H]TdR incorporation) using purified T cells stimulated via CD3 or CD2 pathways.
  • Comparing CD44 signaling with CD28 signaling.

Main Results:

  • CD44 mAb (H90) enhanced T cell proliferation when a primary activation signal was provided via cross-linked CD3 or CD2.

Related Experiment Videos

  • This proliferation was mediated by interleukin-2 (IL-2) synthesis and IL-2 receptor (IL-2R) expression.
  • CD44 and CD28 signaling pathways were distinct, with synergistic effects observed when both were engaged.

    • Conclusions:

    • CD44 plays a dual role in T cell activation: mediating adhesion and delivering a co-stimulatory signal.
    • CD44 signaling is distinct from CD28 signaling, highlighting a hierarchy of T cell activation pathways.
    • Endothelial cells may utilize CD44-mediated signaling as accessory cells in T cell activation.