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A Quantitative Glycomics and Proteomics Combined Purification Strategy
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Relative versus absolute quantitation in disease glycomics.
Edward S X Moh1, Morten Thaysen-Andersen, Nicolle H Packer
1Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.
Proteomics. Clinical Applications
|February 17, 2015
Summary
Quantifying glycans in biological samples is crucial. This review highlights HPLC/CE and LC-MS/MS methods, favoring relative glycan quantitation for biological insights over absolute methods.
Area of Science:
- Glycomics and Glycobiology
- Biomarker Discovery
- Analytical Chemistry
Background:
- The glycome, composed of glycans attached to proteins, is vital in biological materials like blood and tissue.
- Altered glycan structures, linked to glycosyltransferase activity, have significant biological implications.
- Accurate glycan quantitation is essential for understanding cellular glycosylation.
Purpose of the Study:
- To review common quantitative methods in glycomics, including HPLC/CE and LC-MS/MS.
- To discuss the advantages and disadvantages of absolute versus relative glycome quantitation.
- To evaluate the impact of quantitation strategies on glycomics study conclusions.
Main Methods:
- High-Performance Liquid Chromatography (HPLC) and Capillary Electrophoresis (CE).
- Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
- Analysis of absolute and relative quantitation strategies for glycomics.
Main Results:
- Relative glycan quantitation is often more informative and cost-effective for research than absolute quantitation.
- Absolute quantitation may be valuable in specific clinical glycomics applications.
- Stable isotopic labeling offers advanced multiplexing capabilities for glycomics.
Conclusions:
- Relative quantitation provides key biological insights into glycosylation machinery regulation.
- The choice of quantitation method significantly influences the interpretation of glycomics data.
- Future glycomics research can benefit from advanced techniques like stable isotopic labeling for enhanced analysis.
