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Shootward Movement of CFDA Tracer Loaded in the Bottom Sink Tissues of Arabidopsis
Published on: May 11, 2019
Channel characteristics of VDAC-3 from Arabidopsis thaliana
C Berrier1, R Peyronnet2, J-M Betton3
1Neuroscience Paris-Saclay Institute (Neuro-PSI) UMR 9197, CNRS-Université Paris-Sud, Bâtiment 430, F-91405 Orsay Cedex, France.
Abstract:
Four different isoforms of the Voltage-Dependent Anion Channel (VDAC) have been identified in Arabidopsis plant cells. The electrophysiological characteristics of several VDAC channels from animal as well as plant cells are well documented, but those of this model plant are unknown. One isoform, AtVDAC-3 was obtained either directly by cell-free synthesis or produced in Escherichia coli, as inclusion bodies, and re-natured. An electrophysiological study of the purified proteins in planar lipid bilayers showed that both methods yielded proteins with similar channel activity. The characteristics of AtVDAC-3 are that of a bona fide VDAC-like channel.
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