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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

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Specific light-up bioprobes based on AIEgen conjugates.

Jing Liang¹, Ben Zhong Tang, Bin Liu

¹Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore (NUS), Singapore 117585.

Chemical Society Reviews

|February 18, 2015

Summary

Aggregation-induced emission (AIE) fluorogens overcome limitations of traditional probes, enabling sensitive and specific light-up bioprobes for advanced optical sensing and imaging applications.

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Area of Science:

Biomedical Engineering
Optical Imaging
Materials Science

Background:

Conventional fluorescent probes face limitations like aggregation-caused quenching, hindering sensitivity and labeling efficiency.
Aggregation-induced emission (AIE) fluorogens offer a solution by exhibiting enhanced emission upon aggregation.

Purpose of the Study:

To review recent advancements in AIEgen-based light-up bioprobes.
To provide guidelines for designing novel AIE sensing and imaging platforms.

Main Methods:

Functionalization of AIE fluorogens (AIEgens) with specific recognition elements.
Development of light-up probes with activatable therapeutic effects.

Main Results:

AIEgen-based probes demonstrate low background interference and high signal-to-noise ratios.
These probes exhibit superior photostability and activatable therapeutic effects.

Conclusions:

AIEgen-based light-up bioprobes represent a significant advancement for biomedical sensing and imaging.
Future designs should focus on high selectivity, sensitivity, and broad adaptability for diverse applications.