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A sensitive glucose biosensor based on Ag@C core-shell matrix.

Xuan Zhou¹, Xingxin Dai¹, Jianguo Li¹

¹College of Chemistry, Chemical engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, PR China.

Materials Science & Engineering. C, Materials for Biological Applications

|February 18, 2015

Summary

Carbon-coated silver nanoparticles (Ag@C) enhance biosensor performance by improving biocompatibility and stability. This novel core-shell structure enables sensitive and stable glucose detection, paving the way for advanced enzyme biosensors.

Keywords:

Ag@C core–shell structure Biosensor Electrochemistry Glucose oxidase

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Area of Science:

Nanomaterials Science
Electrochemistry
Biosensor Technology

Background:

Silver nanoparticles (Ag) possess unique properties but often suffer from poor biocompatibility and chemical stability.
Developing stable and biocompatible nanomaterials is crucial for effective biosensor development.
Carbon coating offers a promising strategy to enhance nanoparticle properties.

Purpose of the Study:

To synthesize and characterize carbon-coated silver nanoparticles (Ag@C) with improved biocompatibility and stability.
To develop a novel glucose biosensor utilizing the Ag@C core-shell structure for enzyme immobilization.
To evaluate the electrochemical performance and stability of the developed glucose biosensor.

Main Methods:

Synthesis of Ag@C core-shell nanoparticles.
Characterization using Transmission Electron Microscopy (TEM) and Fourier Transform Infrared (FTIR) spectroscopy.
Immobilization of glucose oxidase (GOD) onto Ag@C modified glassy carbon electrode (GCE).
Electrochemical detection of glucose using cyclic voltammetry and amperometry.

Main Results:

TEM confirmed the core-shell structure of Ag@C nanoparticles.
FTIR analysis indicated the presence of functional groups (-OH, -COOH) on the carbon shell, facilitating enzyme immobilization.
The GOD-Ag@C/Nafion/GCE biosensor demonstrated a linear response to glucose from 0.05 to 2.5 mM.
A low detection limit of 0.02 mM (S/N=3) and a high apparent Michaelis-Menten constant (K_M^(app)) of 1.7 mM were achieved.
The biosensor exhibited excellent reproducibility and long-term stability.

Conclusions:

The Ag@C core-shell structure provides a favorable microenvironment for glucose oxidase immobilization and direct electrochemistry.
The developed glucose biosensor shows high sensitivity, affinity, and stability, attributed to the unique properties of the Ag@C matrix.
Core-shell structured Ag@C nanoparticles are ideal matrices for constructing sensitive and stable enzyme biosensors.