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Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
Published on: December 21, 2016
Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus
Filipa A C Soares1, Roger A Pedersen2, Ludovic Vallier2,3
1Anne McLaren Laboratory for Regenerative Medicine and Department of Surgery, Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, West Forvie Building, Robison Way, CB2 0SZ, Cambridge, UK. facs2@cam.ac.uk.
Abstract:
This protocol describes the efficient isolation of peripheral blood mononuclear cells from circulating blood via density gradient centrifugation and subsequent generation of integration-free human induced pluripotent stem cells. Peripheral blood mononuclear cells are cultured for 9 days to allow expansion of the erythroblast population. The erythroblasts are then used to derive human induced pluripotent stem cells using Sendai viral vectors, each expressing one of the four reprogramming factors Oct4, Sox2, Klf4, and c-Myc.
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