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Updated: Apr 17, 2026
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
Published on: May 23, 2018
Improved tagmentation-based whole-genome bisulfite sequencing for input DNA from less than 100 mammalian cells
Hanlin Lu1, Zhimei Yuan, Tao Tan
1BGI-Shenzhen, Shenzhen, China.
Aim:
To develop a whole-genome methylation sequencing method that fulfills the needs for studies using ultra-low-input DNA.
Materials & Methods:
The tagmentation-based whole-genome bisulfite sequencing (T-WGBS) technology is modified, enabling stable library construction with complexity from minimally 0.5 ng of initial genomic DNA, which equals less than 100 mammalian cells.
Results:
We thoroughly assessed the performance of this T-WGBS method by sequencing the methylomes of a rice strain and pre-implantation embryos of rhesus monkey and compare to traditional WGBS approach, thereby demonstrating the efficacy of this new approach.
Conclusion:
This new approach is highly attractive for the complete methylome analysis of very few cells, for example, mammalian pre-implantation embryos, or tiny human biopsy specimens.
