Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.2K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.2K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

16.6K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
16.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rising threats of ceftolozane-tazobactam resistance in P. aeruginosa and A. baumannii: Species-, phenotype-, and geography-specific insights from a systematic review and meta-analysis.

Indian journal of medical microbiology·2026
Same author

Identification, Biology, and Bactericide Control of Peach Bacterial Shot Hole in Hebei Province, China.

Microorganisms·2026
Same author

Actin-dependent regulation of RSV F-mediated cell-cell fusion revealed by visualizing its spatiotemporal dynamics.

Nanoscale horizons·2026
Same author

Targeting Lactate-Driven Stromal Autophagy via MCT1 Disrupts the Immunosuppressive Niche and Sensitizes Pancreatic Cancer to PD-1 Blockade.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

A decade of air pollution in Anhui province: spatiotemporal patterns, and health risks.

International journal of biometeorology·2026
Same author

[Hemostatic efficacy of tranexamic acid combined with absorbable fluid hemostat in single-level transforaminal lumbar interbody fusion during the perioperative period].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2026

Related Experiment Video

Updated: Apr 17, 2026

Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains
07:42

Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains

Published on: July 20, 2022

3.6K

Magnetic-field-enabled resolution enhancement in super-resolution imaging.

Min Zhang1, Junling Chen, Jing Gao

  • 1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China. hdwang@ciac.ac.cn.

Physical Chemistry Chemical Physics : PCCP
|February 18, 2015
PubMed
Summary

Researchers developed a new method to enhance super-resolution fluorescence imaging using magnetic fields. This technique improves the brightness and precision of imaging organic dyes, leading to clearer biological visualizations.

More Related Videos

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.7K
Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

691

Related Experiment Videos

Last Updated: Apr 17, 2026

Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains
07:42

Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains

Published on: July 20, 2022

3.6K
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.7K
Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

691

Area of Science:

  • Biophysics
  • Optical Imaging
  • Materials Science

Background:

  • Super-resolution fluorescence imaging allows visualization of cellular structures below the diffraction limit.
  • Organic dyes are crucial probes in fluorescence imaging but can suffer from photobleaching and low signal intensity.
  • Modulating dye properties is key to advancing imaging capabilities.

Purpose of the Study:

  • To investigate the use of external magnetic fields to modulate the photophysics of organic dyes.
  • To enhance the performance of super-resolution fluorescence imaging techniques like direct stochastic optical reconstruction microscopy (dSTORM).
  • To improve localization precision and overall imaging resolution.

Main Methods:

  • Utilized organic dyes as fluorescent probes in super-resolution imaging experiments.
  • Applied an external magnetic field during imaging acquisition.
  • Quantitatively analyzed changes in fluorescence intensity, probe localization, and photon emission per molecule.
  • Compared imaging results obtained with and without the magnetic field.

Main Results:

  • The external magnetic field significantly increased fluorescence intensity of the organic dyes.
  • A higher number of probe molecules were localized per image under magnetic field application.
  • The number of photons emitted per molecule increased, indicating enhanced photophysical properties.
  • Direct stochastic optical reconstruction microscopy (dSTORM) demonstrated improved localization precision and resolution.

Conclusions:

  • External magnetic fields offer a novel strategy for modulating organic dye photophysics.
  • Magnetic field application enhances key parameters in super-resolution fluorescence imaging.
  • This approach leads to improved imaging resolution and precision, advancing biological visualization.