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Updated: Apr 17, 2026

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells
Published on: June 30, 2023
High-throughput screening approaches to identify regulators of mammalian autophagy
Justin Joachim1, Ming Jiang2, Nicole C McKnight3
1London Research Institute, Cancer Research UK, Secretory Pathway Laboratory, 44 Lincolns Inn Fields, London, WC2A 3LY, UK.
Abstract:
This article discusses the issues to consider in the development and implementation of high-throughput screens (HTSs) using both siRNA libraries and small molecule compound collections, in order to discover autophagy regulators in mammalian cells. We discuss how to develop the screen, focusing on the key parameters to establish in order to perform a successful screen. As our understanding of autophagy increases and its impact on human disease is elucidated, this technology can be further exploited to uncover novel genes, which may one day become new therapeutic targets.
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