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Updated: Apr 17, 2026

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Published on: June 9, 2017
The actin depolymerizing factor (ADF)/cofilin signaling pathway and DNA damage responses in cancer
Chun-Yuan Chang1, Jyh-Der Leu2, Yi-Jang Lee3,4
1Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei 112, Taiwan. tracysirotan@hotmail.com.
Abstract:
The actin depolymerizing factor (ADF)/cofilin protein family is essential for actin dynamics, cell division, chemotaxis and tumor metastasis. Cofilin-1 (CFL-1) is a primary non-muscle isoform of the ADF/cofilin protein family accelerating the actin filamental turnover in vitro and in vivo. In response to environmental stimulation, CFL-1 enters the nucleus to regulate the actin dynamics. Although the purpose of this cytoplasm-nucleus transition remains unclear, it is speculated that the interaction between CFL-1 and DNA may influence various biological responses, including DNA damage repair. In this review, we will discuss the possible involvement of CFL-1 in DNA damage responses (DDR) induced by ionizing radiation (IR), and the implications for cancer radiotherapy.
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