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Related Concept Videos

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The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
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Related Experiment Video

Updated: Apr 17, 2026

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
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Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

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Membrane trafficking: ER export encounters dualism.

Charles Barlowe1

  • 1Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.

Current Biology : CB
|February 19, 2015
PubMed
Summary

The COPII coat complex ensures efficient protein export from the endoplasmic reticulum by recognizing signals on both sorting receptors and their cargo.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Trafficking

Background:

  • Cytoplasmic coat protein complexes are crucial for protein sorting within the endomembrane system.
  • Efficient protein transport is essential for cellular function and homeostasis.

Purpose of the Study:

  • To elucidate the mechanism by which the COPII coat mediates protein export from the endoplasmic reticulum.
  • To investigate the role of dual signal recognition in cargo selection by COPII.

Main Methods:

  • The study likely employed techniques such as co-immunoprecipitation, in vitro binding assays, and possibly cryo-electron microscopy to visualize coat-cargo interactions.
  • Analysis of specific sorting receptor and cargo protein interactions with COPII components.

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Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

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Related Experiment Videos

Last Updated: Apr 17, 2026

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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
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Main Results:

  • The COPII coat utilizes a dual recognition mechanism, binding to signals on both the sorting receptor and the cargo protein.
  • This dual recognition enhances the efficiency and specificity of protein sorting for endoplasmic reticulum export.

Conclusions:

  • The findings reveal a sophisticated mechanism for cargo selection by the COPII coat.
  • This dual recognition system ensures accurate protein trafficking, preventing errors in the endomembrane system.