A new bisphenol A derivative for estrogen receptor binding studies with surface plasmon resonance

A new bisphenol A (BPA) derivative was synthesized and immobilized onto the surface of a CM5 sensor chip for a binding study with orphan estrogen-related receptor γ using the surface plasmon resonance technique. The kinetic parameters, including dissociation and association constants and dissociation and association rate constants, were investigated. Moreover, a competitive inhibition assay was conducted to understand the competition relationship between the free BPA molecules in solution and the immobilized BPA molecules on the sensor chip surface. The CM5 sensor chip immobilized with the BPA derivative can be regenerated by using a high-concentration running buffer for repeated use more than 150 times without any adverse effect on its performance in the binding studies. The results indicate that the system has potential for further development as a sensitive surface plasmon resonance-based biosensor for endocrine-disrupting chemicals.