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Updated: Sep 2, 2026
Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
Published on: October 29, 2018
High-throughput data integration of RNA-miRNA-circRNA reveals novel insights into mechanisms of
Florian Caiment1, Stan Gaj2, Sandra Claessen2
1Department of Toxicogenomics, Maastricht University, Maastricht 6200, The Netherlands florian.caiment@maastrichtuniversity.nl.
Abstract:
The chain of events leading from a toxic compound exposure to carcinogenicity is still barely understood. With the emergence of high-throughput sequencing, it is now possible to discover many different biological components simultaneously. Using two different RNA libraries, we sequenced the complete transcriptome of human HepG2 liver cells exposed to benzo[a]pyrene, a potent human carcinogen, across six time points. Data were integrated in order to reveal novel complex chemical-gene interactions. Notably, we hypothesized that the inhibition of MGMT, a DNA damage response enzyme, by the over-expressed miR-181a-1_3p induced by BaP, may lead to liver cancer over time.
