Related Experiment Video

Updated: Apr 17, 2026

Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors
09:12

Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors

Published on: April 5, 2024

5.0K

Viral clearance using traditional, well-understood unit operations: session 1.2. Anion exchange chromatography; and

David Roush1

  • 1Merck, Sharp, and Dohme, BioProcess Development, 2015 Galloping Hill Road, Mailstop K15-2-H206, Kenilworth, NJ 07033 david_roush@merck.com.

PDA Journal of Pharmaceutical Science and Technology
|February 19, 2015
PubMed
Abstract

No abstract available in PubMed .

Keywords:
AEXAffinityBVDVCHOPCompetitive adsorptionFlowthroughLDAOMMVPP7PartitioningPhagePrVProtein AReoTriton X-100VirusXMuLVφX174

More Related Videos

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation
12:55

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Published on: September 29, 2019

6.4K
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
10:33

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

7.7K

Related Experiment Videos

Last Updated: Apr 17, 2026

Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors
09:12

Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors

Published on: April 5, 2024

5.0K
Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation
12:55

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Published on: September 29, 2019

6.4K
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
10:33

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

7.7K

Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

3.0K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
3.0K
Types Of Column Chromatography01:29

Types Of Column Chromatography

15.3K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
15.3K

Articles linked to this work by shared authors, journal, and citation graph.

Current State and Impact of Implementation of In-Silico Tools in the Biopharmaceutical Industry - Proceedings of the 6th Modeling Workshop.

Biotechnology and bioengineering·2026

Purification of Advanced Therapeutics by Flow-Through Pseudo-Affinity Chromatography: Bi-Specific mABs, Fc Fusion Proteins, and Adeno-Associated Viruses.

Biotechnology and bioengineering·2026

Flow-through polishing of Protein A eluates: Removal of high and low molecular weight impurities by size-exclusion-mixed-mode chromatography.

Journal of chromatography. A·2025

Integration of QSAR models with high throughput screening to accelerate the development of polishing chromatography unit operations.

Journal of chromatography. A·2025

Current state of implementation of in silico tools in the biopharmaceutical industry-Proceedings of the 5th modeling workshop.

Biotechnology and bioengineering·2024

Mixed-mode size-exclusion silica resin for polishing human antibodies in flow-through mode.

Journal of chromatography. A·2024

Quantifying User-Applied Forces on Prefilled Syringe Luer Cone: A Human Factors Ergonomics Study.

PDA journal of pharmaceutical science and technology·2026

Analytical Characterization of Polymer Components in Parenteral Primary Packaging.

PDA journal of pharmaceutical science and technology·2026

CQV Considerations for Single-Use Systems in Biopharmaceutical Manufacturing: A Practical Framework.

PDA journal of pharmaceutical science and technology·2026

Water Activity as a Primary Indicator of Moisture in Lyophilized Products: Linking Non-Destructive Headspace Analysis to Total Moisture via Moisture Sorption Isotherms.

PDA journal of pharmaceutical science and technology·2026

Combining a Respiration-Based Method with Universal qPCR for 3-Day Rapid Sterility Testing.

PDA journal of pharmaceutical science and technology·2026

Pseudomonas aeruginosa contamination in purified water systems: case study in a pharmaceutical industry.

PDA journal of pharmaceutical science and technology·2026

Non-target screening using fast comprehensive two-dimensional liquid chromatography-high-resolution mass spectrometry I: investigating stability across a set of wastewater samples.

Journal of chromatography. A·2026

Centrifugal mini-SPE cleanup for monitoring contaminants in food. Part I: Comparison with dispersive-SPE.

Journal of chromatography. A·2026

Chiral HPLC in biomedical and pharmaceutical analysis: Method development challenges and application-oriented strategies.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026

Consensus descriptor recurrence across complementary ANN workflows in chiral HPLC: An interaction-domain analysis.

Talanta·2026

Impact of Salt and Urine Matrices on Electrokinetic Stacking Coupled to Paper Spray Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026

Fluorous Affinity-Driven Retention of Fluorinated Analytes on a High-Density Fluorinated Silica Gel Stationary Phase.

Analytical chemistry·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us