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Updated: Jun 25, 2026
Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse
Published on: October 26, 2017
Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy
Lingmin Mu1, Changqin Jing2, Zhikun Guo3
1Morphological Laboratory of Xinxiang Medical University, Xinxiang, Henan 453003, P. R. China.
Insights
Increased CD11a, CD11b, and CD11c integrin expressions are linked to myocardial hypertrophy in rats. This finding suggests a role for these integrins in the development of heart enlargement and offers potential targets for intervention.
Area of Science:
- Cardiovascular Biology
- Integrin Signaling
- Pathology
Background:
- Myocardial hypertrophy is a significant risk factor for cardiovascular diseases.
- Integrins are cell surface receptors involved in cell adhesion and signaling.
- Understanding the molecular mechanisms of myocardial hypertrophy is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the expression of CD11a, CD11b, and CD11c integrins in rat myocardial hypertrophy.
- To explore the role of these integrins in the pathogenesis of cardiac enlargement.
- To provide morphological and molecular data for understanding hypertrophy and its potential reversal.
Main Methods:
- Established isoproterenol-induced myocardial hypertrophy in Sprague-Dawley rats.
- Analyzed myocardial tissue using hematoxylin staining for morphological changes.
- Quantified CD11a, CD11b, and CD11c integrin expression via immunohistochemistry and RT-PCR.
Main Results:
- Significantly elevated protein expression of CD11a, CD11b, and CD11c integrins in hypertrophied myocardial tissue.
- mRNA expression levels of these integrins corroborated immunohistochemical findings.
- Consistent quantitative increases in integrin expression were observed.
Conclusions:
- Increased expression of CD11a, CD11b, and CD11c integrins is associated with myocardial hypertrophy.
- These integrins may play a critical role in the development of cardiac enlargement.
- The findings suggest potential therapeutic targets for managing myocardial hypertrophy.
Objectives:
To examine the expressions of CD11a, CD11b, and CD11c integrins in the myocardial tissues of rats with isoproterenol-induced myocardial hypertrophy. This study also provided morphological data to investigate the signal transduction mechanisms of myocardial hypertrophy and reverse it.
Materials And Methods:
A myocardial hypertrophy model was established by subcutaneously injecting isoprenaline in healthy adult Sprague-Dawley rats. Myocardial tissues were obtained, embedded in conventional paraffin, sectioned, and stained with hematoxylin. Pathological changes in myocardial tissues were then observed. The expressions and distributions of CD11a, CD11b, and CD11c integrins were detected by immunohistochemistry. Changes in the mRNA expressions of CD11a, CD11b, and CD11c in the myocardial tissues of rats were detected by RT-PCR. Image analysis software was used to determine the expressions of CD11a, CD11b, and CD11c integrins quantitatively.
Results:
Immunohistochemical results showed that the positive expressions of CD11a, CD11b, and CD11c integrins increased significantly in the experimental group compared with those in the control group. The mRNA expressions of CD11a, CD11b, and CD11c in the myocardial tissues of rats were consistent with the immunohistochemical results.
Conclusion:
The increase in the protein expressions of CD11a, CD11b, and CD11c integrins may have an important role in the occurrence and development of myocardial hypertrophy.
