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AKT family and miRNAs expression in IL-2 induced CD4(+)T cells

Najmeh Ranji1, Majid Sadeghizadeh2, Morteza Karimipoor3

  • 1Department of Genetics, College of Science, Rasht Branch, Islamic Azad University, Rasht, Iran.

Iranian Journal of Basic Medical Sciences
|February 19, 2015
PubMed
Abstract

Insights

MicroRNAs (miRNAs) regulate PI3K/AKT signaling and apoptosis in activated CD4(+) T cells. Upregulated miRNAs in IL-2 depleted cells likely control AKT3 decrease, impacting T cell function.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Non-coding RNAs, specifically microRNAs (miRNAs), are crucial for biological processes and therapeutic development.
  • The precise roles of miRNAs in PI3K/AKT signaling and apoptosis following T cell activation remain incompletely understood.

Purpose of the Study:

  • To investigate miRNA expression and identify their targets within the PI3K/AKT signaling and apoptosis pathways in activated CD4(+) T cells.
  • To elucidate the functional impact of miRNAs on T cell proliferation and IL-2 secretion.

Main Methods:

  • Quantitative RT-PCR was used to measure miRNA expression and target gene levels.
  • Bioinformatics approaches predicted potential miRNA targets in relevant signaling pathways.
  • Cell proliferation and IL-2 secretion assays assessed T cell responses to IL-2 treatment.

Main Results:

  • Interleukin-2 (IL-2) treatment dose-dependently increased CD4(+) T cell proliferation and IL-2 secretion.
  • Bioinformatic analysis identified AKT1, AKT3, and apoptotic genes as putative targets of upregulated miRNAs.
  • Quantitative RT-PCR confirmed decreased AKT3 expression in IL-2 depleted cells, suggesting miRNA-mediated regulation.

Conclusions:

  • Specific miRNAs are likely upregulated in IL-2 depleted CD4(+) T cells, leading to decreased AKT3 expression.
  • These findings suggest a role for miRNAs in regulating apoptosis pathways in both IL-2-induced and IL-2-uninduced T cells.
Keywords:
AKT3IL-2 inductionIn silico analysismiRNAs

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