Related Experiment Video
Updated: Apr 17, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Regularities of Anthocyanins Retention in RP HPLC for "Water-Acetonitrile-Phosphoric Acid" Mobile Phases
V I Deineka1, L A Deineka1, I I Saenko1
1Faculty of Biology and Chemistry, Federal State Autonomous Educational Institution of Higher Professional Education "Belgorod National Research University", Pobeda Street 85, Belgorod 308015, Russia.
Abstract:
The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis. The differences and similarities of anthocyanins separation were revealed. It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Chromatographic Methods: Terminology
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Instrumentation
Analyte Adsorption and Distribution

