Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

13.8K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
13.8K
Proofreading01:43

Proofreading

62.7K
Overview
62.7K
Proofreading01:31

Proofreading

10.0K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Challenges in Multilingual Adverse Drug Reaction Detection on Social Media: Insights from Case Studies.

Studies in health technology and informatics·2025
Same author

Distinct Substrates of Idiopathic Ventricular Fibrillation Revealed by Arrhythmia Characteristics on Implantable Cardioverter-Defibrillator.

JACC. Clinical electrophysiology·2024
Same author

iMab antibody binds single-stranded cytosine-rich sequences and unfolds DNA i-motifs.

Nucleic acids research·2024
Same author

Publisher Correction: Remotely sensing potential climate change tipping points across scales.

Nature communications·2024
Same author

Remotely sensing potential climate change tipping points across scales.

Nature communications·2024
Same author

Unprecedented reactivity of polyamines with aldehydic DNA modifications: structural determinants of reactivity, characterization and enzymatic stability of adducts.

Nucleic acids research·2023

Related Experiment Video

Updated: Apr 17, 2026

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

2.0K

Construction of anti-parallel G-quadruplexes through sequential templated click.

Romaric Bonnet1, Thomas Lavergne, Béatrice Gennaro

  • 1Université Grenoble Alpes, Département de Chimie Moléculaire, CNRS UMR 5250, F-38000 Grenoble, France. thomas.lavergne@ujf-grenoble.fr eric.defrancq@ujf-grenoble.fr.

Chemical Communications (Cambridge, England)
|February 20, 2015
PubMed
Summary

Researchers created DNA-peptide conjugates that fold into stable G-quadruplex structures. These structures show reduced structural variations, advancing DNA nanotechnology and drug design.

More Related Videos

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.4K
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
12:31

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

26.2K

Related Experiment Videos

Last Updated: Apr 17, 2026

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

2.0K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.4K
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
12:31

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

26.2K

Area of Science:

  • Bioconjugation Chemistry
  • Supramolecular Chemistry
  • Nucleic Acid Chemistry

Background:

  • Cyclopeptide scaffolds offer unique structural properties for biomolecule assembly.
  • DNA G-quadruplexes are G-rich nucleic acid structures with significant biological relevance.
  • Controlling the higher-order structure of DNA is crucial for applications in nanotechnology and therapeutics.

Purpose of the Study:

  • To develop a method for assembling biologically relevant DNA sequences onto addressable cyclopeptide scaffolds.
  • To investigate the structural properties and stability of the resulting DNA-peptide conjugates.
  • To explore the potential of these conjugates in forming well-defined DNA G-quadruplex structures.

Main Methods:

  • Sequential oxime and copper-catalyzed azide-alkyne cycloaddition (CuAAc) reactions were employed for conjugation.
  • Biologically relevant DNA sequences were synthesized and functionalized for attachment.
  • Addressable cyclopeptide scaffolds were utilized as platforms for DNA assembly.

Main Results:

  • Successful assembly of DNA sequences onto cyclopeptide scaffolds was achieved.
  • The resulting conjugates demonstrated the ability to fold into well-defined anti-parallel DNA G-quadruplex structures.
  • These G-quadruplex structures exhibited high stability and reduced polymorphism compared to conventional G-quadruplexes.

Conclusions:

  • The developed conjugation strategy enables the formation of stable, well-defined DNA G-quadruplex structures on cyclopeptide scaffolds.
  • These findings provide a novel platform for designing complex DNA architectures with enhanced structural integrity.
  • The study opens avenues for the application of these DNA-peptide conjugates in areas such as diagnostics, therapeutics, and nanomaterials.