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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Updated: Apr 17, 2026

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

Methods in molecular cardiology: microarray technology.

B van den Bosch, P A Doevendans, D Lips

Netherlands Heart Journal : Monthly Journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation

|February 20, 2015

Summary

This summary is machine-generated.

Gene expression changes are crucial in cardiovascular diseases. DNA microarray technology helps identify gene expression patterns for understanding disease mechanisms and developing diagnostics.

Keywords:

microarray technology molecular cardiology

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Last Updated: Apr 17, 2026

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

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Published on: July 3, 2018

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Published on: December 3, 2010

Area of Science:

Molecular Biology
Cardiovascular Research

Background:

Gene expression alterations are implicated in cardiovascular diseases, including myocardial hypertrophy and pump failure.
Understanding gene expression profiles offers insights into disease pathophysiology and potential therapeutic targets.
Gene activity patterns can be utilized for diagnostic and prognostic classifications of cardiovascular conditions.

Approach:

DNA microarray technology enables simultaneous analysis of numerous gene expressions in a single assay.
Messenger RNA (mRNA) levels, indicative of gene activity, are quantified using fluorescently labeled nucleotides (Cy3/Cy5) for test and reference samples.
Signal intensities and ratios of fluorescent groups on the microarray determine relative gene transcript amounts.

Key Points:

Development of in-house DNA microarray technology using commercially available cDNA collections.
Comparison of various technical approaches, highlighting potential pitfalls and possibilities.
Application of microarray technology to discern gene expression differences in normal, hypertrophic, and failing heart conditions.

Conclusions:

This study details the establishment and evaluation of an in-house DNA microarray system.
The developed technology facilitates the investigation of gene expression in cardiovascular disease contexts.
Findings contribute to understanding gene expression in normal versus diseased heart states.