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Updated: Apr 17, 2026

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Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord
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Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging
Amnon Bar-Shir1, Yajie Liang, Kannie W Y Chan
1Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Abstract:
Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

