Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Necrosis01:16

Necrosis

7.4K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
7.4K
Oxymercuration-Reduction of Alkenes02:36

Oxymercuration-Reduction of Alkenes

10.1K
Oxymercuration–reduction of alkenes is one of the major reactions converting alkenes to alcohols. It involves the hydration of alkenes with mercuric acetate in a mixture of tetrahydrofuran and water, forming an organomercury adduct. This is followed by a demercuration step in which the adduct is reduced to an alcohol using sodium borohydride.
10.1K
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

3.3K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
3.3K
Nitriles to Ketones: Grignard Reaction00:57

Nitriles to Ketones: Grignard Reaction

7.9K
Organomagnesium halides, commonly known as Grignard reagents, convert nitriles to ketones and proceed through a nucleophilic acyl substitution. Nitriles react with a Grignard reagent, followed by an aqueous acid, to yield ketones. The reaction introduces a new carbon–carbon bond. The alkyl–magnesium bond in the Grignard reagent is highly polar, so the alkyl carbon develops a carbanionic character and acts as a nucleophile.
The mechanism begins with a nucleophilic attack by the...
7.9K
Radical Reactivity: Nucleophilic Radicals01:16

Radical Reactivity: Nucleophilic Radicals

2.8K
Radicals adjacent to electron-donating groups are called nucleophilic radicals. These radicals readily react with electrophilic alkenes. The SOMO–LUMO interactions are the driving force for the reaction, where the high-energy SOMO of the electron-rich, nucleophilic radicals interacts with the low-energy LUMO of the electron-deficient, electrophilic alkenes. Such SOMO–LUMO interactions are the basis of reactive radical traps, affecting the selectivity in radical reactions. For...
2.8K
Preparation of Epoxides03:00

Preparation of Epoxides

10.0K
Overview
Epoxides result from alkene oxidation, which can be achieved by a) air, b) peroxy acids, c) hypochlorous acids, and d) halohydrin cyclization.
Epoxidation with Peroxy Acids
Epoxidation of alkenes via oxidation with peroxy acids involves the conversion of a carbon–carbon double bond to an epoxide using the oxidizing agent meta-chloroperoxybenzoic acid, commonly known as MCPBA. Since the O–O bond of peroxy acids is very weak, the addition of electrophilic oxygen of peroxy...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic modification of the marine-derived yeast Yarrowia lipolytica with high-protein content using a GPI-anchor-fusion expression system.

Biotechnology progress·2009
Same author

Regulation of the Edwardsiella tarda hemolysin gene and luxS by EthR.

Journal of microbiology and biotechnology·2009
Same author

EBV LMP2A-specific T cell immune responses elicited by dendritic cells loaded with LMP2A protein.

Cellular & molecular immunology·2009
Same author

[Combination of volar buttress plate with external fixator for the distal radial fractures of type C3 caused by high-energy injuries].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2009
Same author

Environmental regulation of floral anthocyanin synthesis in Ipomoea purpurea.

Molecular ecology·2009
Same author

PI3K integrates the effects of insulin and leptin on large-conductance Ca2+-activated K+ channels in neuropeptide Y neurons of the hypothalamic arcuate nucleus.

American journal of physiology. Endocrinology and metabolism·2009

Related Experiment Video

Updated: Apr 17, 2026

Line Shape Analysis of Dynamic NMR Spectra for Characterizing Coordination Sphere Rearrangements at a Chiral Rhenium Polyhydride Complex
10:52

Line Shape Analysis of Dynamic NMR Spectra for Characterizing Coordination Sphere Rearrangements at a Chiral Rhenium Polyhydride Complex

Published on: July 27, 2022

3.5K

Necroptosis-inducing rhenium(V) oxo complexes.

Kogularamanan Suntharalingam1, Samuel G Awuah, Peter M Bruno

  • 1Department of Chemistry, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.

Journal of the American Chemical Society
|February 21, 2015
PubMed
Summary

New rhenium complexes kill cancer cells by inducing programmed necrosis (necroptosis), not apoptosis. These compounds show low toxicity in mice and stability in human blood, indicating potential therapeutic applications.

More Related Videos

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
10:51

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

Published on: April 10, 2015

12.9K
Combining Solid-state and Solution-based Techniques: Synthesis and Reactivity of ChalcogenidoplumbatesII or IV
10:42

Combining Solid-state and Solution-based Techniques: Synthesis and Reactivity of ChalcogenidoplumbatesII or IV

Published on: December 29, 2016

11.3K

Related Experiment Videos

Last Updated: Apr 17, 2026

Line Shape Analysis of Dynamic NMR Spectra for Characterizing Coordination Sphere Rearrangements at a Chiral Rhenium Polyhydride Complex
10:52

Line Shape Analysis of Dynamic NMR Spectra for Characterizing Coordination Sphere Rearrangements at a Chiral Rhenium Polyhydride Complex

Published on: July 27, 2022

3.5K
The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
10:51

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

Published on: April 10, 2015

12.9K
Combining Solid-state and Solution-based Techniques: Synthesis and Reactivity of ChalcogenidoplumbatesII or IV
10:42

Combining Solid-state and Solution-based Techniques: Synthesis and Reactivity of ChalcogenidoplumbatesII or IV

Published on: December 29, 2016

11.3K

Area of Science:

  • Inorganic Chemistry
  • Medicinal Chemistry
  • Cancer Biology

Background:

  • Cancer cell death mechanisms are crucial for therapeutic development.
  • Non-apoptotic cell death pathways like necroptosis offer alternative strategies.
  • Rhenium complexes have shown promise in various biomedical applications.

Purpose of the Study:

  • To synthesize and characterize novel rhenium(V) oxo complexes.
  • To investigate the mechanism of cancer cell death induced by these complexes.
  • To evaluate the in vivo toxicity and stability of the promising compounds.

Main Methods:

  • Synthesis of rhenium(V) oxo complexes with 4,7-diphenyl-1,10-phenanthroline and 3,4,7,8-tetramethyl-1,10-phenanthroline ligands.
  • Assessment of cancer cell viability and induction of necroptosis.
  • Measurement of intracellular reactive oxygen species (ROS) and mitochondrial membrane potential.
  • Evaluation of acute toxicity in C57BL/6 mice and stability in human blood.

Main Results:

  • Two rhenium complexes ([ReO(OMe)(N^N)Cl2]) effectively killed cancer cells.
  • Cell death was mediated by necroptosis, characterized by RIP1-RIP3-dependent ROS production and propidium iodide uptake.
  • Mitochondrial membrane potential depletion was observed, likely downstream of ROS.
  • The complexes exhibited low acute toxicity in mice and good stability in human blood.

Conclusions:

  • Rhenium complexes 1 and 2 are the first to induce necroptosis in cancer cells.
  • These findings highlight a novel mechanism for rhenium-based anticancer agents.
  • The favorable toxicity and stability profiles suggest potential for further therapeutic development.